Two different procedures for the methylation of fatty acids (FAs) and trans fatty acids (TFAs) in food fats were compared using gas chromatography (GC-FID). Gas Chromatography Gustavo Serrano, Ph.D. × 0.25 µm,Omegawax 320 capillary column: 30 m × 0.32 mm i.d. × 0.2 µm,DB-1 capillary column: 30 m × 0.32 mm i.d. The accuracies of said methodologies were tested via comparison with the AOCS standard method, whereas their reproducibility was assessed by analysis of variance of (replicated) data. All rights reserved. The analysis of fatty acids in the free form instead of as fatty acid methyl esters results in easier and quicker sample preparation. Reducing their polarity can make them more amenable for analysis (,This section provides a brief overview of many GC techniques used in this field. For Permissions, please email: [email protected],Systematic Error for Extraction of Controlled Substances from Plant/Fungal Materials,Impurity Profiling of a Novel Anti-MRSA Antibacterial Drug: Alalevonadifloxacin,Novel Eco-friendly HPLC Methods Using Refractive Index Detector for Analysis of Three Veterinary Antibiotics in Pharmaceutical Formulations and Rat Plasma,Advances in Separation and Purification of Bioactive Polysaccharides through High-speed Counter-Current Chromatography,Clarification on “Critical Comments on ‘Assessment of the Thermodynamic Properties of DL-p-Mentha-1,8-diene, 4-isopropyl-1-methylcyclohexene (DL-Limonene) by Inverse Gas Chromatography (IGC)’”,Receive exclusive offers and updates from Oxford Academic.DB-WAX capillary column: 30 m × 0.25 mm i.d.60°C, 1 min, 50°C/min to 175°C, 6.5 min, 2.5°C/min to 200°C, 2 min, 15°C/min to 255°C, 8 min.HP-5 capillary column: 50 m × 0.32 mm i.d.50°C, 1 min, 30°C/min to 150°C, 2°C/min to 250°C, 5°C/min to 300°C,DB wax capillary column: 30 m × 0.25 mm i.d.× 0.25 µm,DB-5 capillary column: 30 m × 0.25 mm i.d.× 0.25 µm,65°C, 2 min, 20°C/min to 165°C, 0.4 min, 20°C/min to 215°C, 6.6 min, 5°C/min to 260°C, 10 min,CP-Sil 88 capillary column: 50 m × 0.32 mm i.d.× 0.2 µm,60°C, 1 min, 25°C/min to 160°C, 28 min, 25°C/min to 190°C, 17 min, 25°C/min to 220°C, 10 min,J&W DB 23 capillary column: 30 m × 0.25 mm i.d.× 0.25 µm,153°C, 2 min, 2.3°C/min to 174°C, 2°C/min to 220°C, 2 min,19091B-102 capillary column: 25 m × 0.2 mm i.d.× 0.33 µm,170°C, 28°C/min to 288°C, 60°C/min to 310°C, 1.25 min,Omegawax 320 capillary column: 30 m × 0.32 mm i.d.× 0.25 µm,80°C, 1.5 min, 40°C/min to 160°C, 3°C/min to 240°C, 12 min,HP-5MS capillary column: 30 m × 0.25 mm i.d. This article also reviews the components of fatty acids in marine animals, marine plants and marine microorganisms.Gas chromatography (GC) has been one of the most widely used commercial analysis techniques for more than half a century. × 0.20 µm,BP-20 capillary column: 60 m × 0.22 mm i.d. Many marine fatty fish species are excellent dietary sources of HUFAs and PUFAs (,In another study, Wetzel and Reynolds reported the results of FA analyses of bowhead whale (.A few thousands of species of marine algae comprise a considerable part of the biomass of the ocean.
ROMANUS, J. POBLOME, K. VERBEKE, A. LUYPAERTS, P. JACOBS, D. DE VOS, M. WAELKENS.AN EVALUATION OF ANALYTICAL AND INTERPRETATIVE METHODOLOGIES FOR THE EXTRACTION AND IDENTIFICATION OF LIPIDS ASSOCIATED WITH POTTERY SHERDS FROM THE SITE OF SAGALASSOS, TURKEY.Danielle Elsey, David Jameson, Barry Raleigh, Michael J. Cooney.Fluorescent measurement of microalgal neutral lipids.Your Mendeley pairing has expired. × 0.25 µm,SolGel-1 capillary column: 30 m × 0.25 mm i.d. : A proposed lipid standard for the algal biofuel industry and its application to diverse taxa for screening lipid content.Development of Gas Chromatography Analysis of Fatty Acids in Marine Organisms.Dulce Martins, Luísa Custódio, Luísa Barreira, Hugo Pereira, Radhouan Ben-Hamadou, João Varela, Khalid Abu-Salah.Alternative Sources of n-3 Long-Chain Polyunsaturated Fatty Acids in Marine Microalgae.Determination of fatty acid methyl esters by GC–triple quadrupole MS using electron and chemical ionization.P. × 0.25 µm,DB-5 capillary column: 30 m × 0.25 mm i.d. A recent report revealed 800 research publications per year under the broad subject of gas chromatography (,The properties of GC are that it is sensitive, rapid and precise, and provides good reproducible analysis; the advent of GC has led to the relatively specific, sensitive and rapid analysis of FAs (,Many marine organisms are known to be excellent dietary sources of highly saturated fatty acids (SFAs) and unsaturated fatty acids (UFAs), and especially of highly unsaturated fatty acids (HUFAs) and polyunsaturated fatty acids (PUFAs) (.The primary purposes of this review are to illustrate the development of GC technologies for the analysis of FAs from marine organisms and to focus on the composition of FAs in different marine organisms.GC is used widely to analyze FAs after derivatization to fatty acid methyl esters (FAMEs), and is used in applications involving the analysis of FAs in marine organisms.
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